PrimePCR™ Probe Assay: GNAI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GNAI1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GNAI1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051459
Assay Design:   exonic
Chromosome Location:   7:79818408-79818512question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000351004 ENST00000418742 ENST00000457358 ENST00000447650

Gene Information

Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha beta and gamma subunits. The alpha subunit binds guanine nucleotide can hydrolyze GTP and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2012]

Gene Symbol:   GNAI1
Gene Name:   guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Aliases:   Gi
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000127955
Entrez:   2770
Chromosome Mapping:   7q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.910000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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