PrimePCR™ Probe Assay: CDKN2D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDKN2D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CDKN2D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051526
Assay Design:   exonic
Chromosome Location:   19:10677802-10677907question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000393599 ENST00000335766

Gene Information

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6 and prevent the activation of the CDK kinases thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation as well as spermatogenesis. Two alternatively spliced variants of this gene which encode an identical protein have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   CDKN2D
Gene Name:   cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
Aliases:   INK4D, p19, p19-INK4D
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000129355
Entrez:   1032
Chromosome Mapping:   19p13

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