PrimePCR™ Probe Assay: UBA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: UBA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UBA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051612
Assay Design:   exonic
Chromosome Location:   X:47058630-47058757question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000360295 ENST00000540632 ENST00000317338 ENST00000442608 ENST00000262052 ENST00000394904 ENST00000377351 ENST00000412206 ENST00000427561 ENST00000442035 ENST00000457753 ENST00000335972 ENST00000451702 ENST00000369864 ENST00000269142 ENST00000578121 ENST00000400466

Gene Information

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   UBA1
Gene Name:   ubiquitin-like modifier activating enzyme 1
Aliases:   A1S9, A1S9T, A1ST, AMCX1, GXP1, MGC4781, POC20, SMAX2, UBA1A, UBE1, UBE1X
RefSeq:   NC_000023.10 NG_012517.1 NG_021353.1 NT_079573.4 NG_009161.1
Ensembl:   ENSG00000130985
Entrez:   7317
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.660000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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