PrimePCR™ Probe Assay: CDC42BPA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDC42BPA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CDC42BPA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052421
Assay Design:   exonic
Chromosome Location:   1:227219029-227219176question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000366769 ENST00000448940 ENST00000442054 ENST00000366765 ENST00000429440 ENST00000441725 ENST00000334218 ENST00000366766 ENST00000366764 ENST00000535525 ENST00000366767

Gene Information

The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described and the full-length nature of two of them has been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   CDC42BPA
Gene Name:   CDC42 binding protein kinase alpha (DMPK-like)
Aliases:   DKFZp686L1738, DKFZp686P1738, FLJ23347, KIAA0451, MRCK, MRCKA, PK428
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000143776
Entrez:   8476
Chromosome Mapping:   1q42.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.630000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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