PrimePCR™ Probe Assay: CDC25C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDC25C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CDC25C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053071
Assay Design:   exonic
Chromosome Location:   5:137665335-137666790question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000348983 ENST00000357274 ENST00000356505 ENST00000510119 ENST00000503022 ENST00000514555 ENST00000513970 ENST00000415130 ENST00000323760

Gene Information

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described however the full-length nature of many of them is not known. [provided by RefSeq Jul 2008]

Gene Symbol:   CDC25C
Gene Name:   cell division cycle 25 homolog C (S. pombe)
Aliases:   CDC25
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000158402
Entrez:   995
Chromosome Mapping:   5q31

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