PrimePCR™ Probe Assay: COL3A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COL3A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COL3A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053821
Assay Design:   exonic
Chromosome Location:   2:189873813-189874905question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000304636 ENST00000317840

Gene Information

This gene encodes the pro-alpha1 chains of type III collagen a fibrillar collagen that is found in extensible connective tissues such as skin lung uterus intestine and the vascular system frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV and with aortic and arterial aneurysms. Two transcripts resulting from the use of alternate polyadenylation signals have been identified for this gene. [provided by R. Dalgleish Feb 2008]

Gene Symbol:   COL3A1
Gene Name:   collagen, type III, alpha 1
Aliases:   EDS4A, FLJ34534
RefSeq:   NC_000002.11 NG_007404.1 NT_005403.17
Ensembl:   ENSG00000168542
Entrez:   1281
Chromosome Mapping:   2q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.460000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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