PrimePCR™ Probe Assay: SLC8A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC8A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC8A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054827
Assay Design:   exonic
Chromosome Location:   2:40657235-40657341question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000406785 ENST00000403092 ENST00000405901 ENST00000402441 ENST00000405269 ENST00000455476 ENST00000448531 ENST00000417271 ENST00000542756 ENST00000332839 ENST00000408028 ENST00000406391 ENST00000542024

Gene Information

In cardiac myocytes Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.[supplied by OMIM Apr 2004]

Gene Symbol:   SLC8A1
Gene Name:   solute carrier family 8 (sodium/calcium exchanger), member 1
Aliases:   DKFZp779F0871, FLJ37694, FLJ43417, MGC119581, NCX1
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000183023
Entrez:   6546
Chromosome Mapping:   2p23-p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.050000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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