PrimePCR™ Probe Assay: HSF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSF1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054981
Assay Design:   exonic
Chromosome Location:   8:145532612-145533141question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000573054 ENST00000568392 ENST00000570268 ENST00000565865 ENST00000406961 ENST00000405141 ENST00000407230 ENST00000395856 ENST00000348332 ENST00000538539 ENST00000288599 ENST00000273371 ENST00000488919 ENST00000495992 ENST00000494440 ENST00000226218 ENST00000559234 ENST00000544979 ENST00000560361 ENST00000373263 ENST00000540170 ENST00000528838 ENST00000533240 ENST00000400780 ENST00000438480 ENST00000457542 ENST00000456763 ENST00000221214 ENST00000260357 ENST00000517685 ENST00000521374 ENST

Gene Information

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90 by itself and/or associated with multichaperone complexes is a major repressor of this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   HSF1
Gene Name:   heat shock transcription factor 1
Aliases:   HSTF1
RefSeq:   NC_000008.10 NT_037704.5 NW_003315924.1
Ensembl:   ENSG00000185122
Entrez:   3297
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.190000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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