PrimePCR™ Probe Assay: NTRK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NTRK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NTRK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055570
Assay Design:   exonic
Chromosome Location:   1:156845344-156845886question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000392302 ENST00000368196 ENST00000524377 ENST00000358660

Gene Information

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that upon neurotrophin binding phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain anhidrosis self-mutilating behavior mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found but only three have been characterized to date. [provided by RefSeq Jul 2008]

Gene Symbol:   NTRK1
Gene Name:   neurotrophic tyrosine kinase, receptor, type 1
Aliases:   DKFZp781I14186, MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA
RefSeq:   NG_016849.1 NC_000001.10 NG_007493.1 NT_004487.19
Ensembl:   ENSG00000198400
Entrez:   4914
Chromosome Mapping:   1q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.230000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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