PrimePCR™ Probe Assay: ARPC4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ARPC4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARPC4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056032
Assay Design:   exonic
Chromosome Location:   3:9845695-9847980question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000263196 ENST00000537045 ENST00000545799 ENST00000307257 ENST00000269967 ENST00000379802 ENST00000418664 ENST00000537688 ENST00000498623 ENST00000397261 ENST00000433034 ENST00000287613 ENST00000383408 ENST00000503781 ENST00000580640 ENST00000302079 ENST00000456430 ENST00000295989 ENST00000595469

Gene Information

This gene encodes one of seven subunits of the human Arp2/3 protein complex. This complex controls actin polymerization in cells and has been conserved throughout eukaryotic evolution. This gene encodes the p20 subunit which is necessary for actin nucleation and high-affinity binding to F-actin. Alternative splicing results in multiple transcript variants. Naturally occurring read-through transcription exists between this gene and the downstream tubulin tyrosine ligase-like family member 3 (TTLL3) which results in the production of a fusion protein. [provided by RefSeq Nov 2010]

Gene Symbol:   ARPC4
Gene Name:   actin related protein 2/3 complex, subunit 4, 20kDa
Aliases:   ARC20, MGC13544, P20-ARC
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000241553
Entrez:   10093
Chromosome Mapping:   3p25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 36.750000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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