PrimePCR™ Probe Assay: LDHA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LDHA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LDHA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057534
Assay Design:   exonic
Chromosome Location:   11:18421091-18422476question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000422447 ENST00000543445 ENST00000396222 ENST00000227157 ENST00000478970 ENST00000495052 ENST00000379412 ENST00000542179 ENST00000540430 ENST00000541669 ENST00000280704 ENST00000537486 ENST00000544105 ENST00000535809

Gene Information

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq Sep 2008]

Gene Symbol:   LDHA
Gene Name:   lactate dehydrogenase A
Aliases:   GSD11, LDH1, LDHM
RefSeq:   NC_000011.9 NG_011816.1 NT_009237.18 NG_008185.1
Ensembl:   ENSG00000134333
Entrez:   3939
Chromosome Mapping:   11p15.4

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