PrimePCR™ Probe Assay: ANAPC10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ANAPC10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ANAPC10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057545
Assay Design:   exonic
Chromosome Location:   4:146017138-146017230question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000507656 ENST00000451299 ENST00000309439 ENST00000514390 ENST00000513054 ENST00000512680 ENST00000504721 ENST00000502847

Gene Information

ANAPC10 is a core subunit of the anaphase-promoting complex (APC) or cyclosome a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836) the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al. 2001 [PubMed 11524682]).[supplied by OMIM Feb 2011]

Gene Symbol:   ANAPC10
Gene Name:   anaphase promoting complex subunit 10
Aliases:   APC10, DKFZp564L0562, DOC1
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164162
Entrez:   10393
Chromosome Mapping:   4q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.850000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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