PrimePCR™ Probe Assay: AKAP13, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AKAP13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AKAP13, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057658
Assay Design:   exonic
Chromosome Location:   15:86262420-86262520question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000394518 ENST00000361243 ENST00000394510

Gene Information

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in at least 3 transcript variants encoding different isoforms containing a dbl oncogene homology (DH) domain and a pleckstrin homology (PH) domain. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway and in addition function as protein kinase A-anchoring proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   AKAP13
Gene Name:   A kinase (PRKA) anchor protein 13
Aliases:   AKAP-Lbc, ARHGEF13, BRX, FLJ11952, FLJ43341, HA-3, Ht31, LBC, PROTO-LB, PROTO-LBC, c-lbc
RefSeq:   NC_000015.9 NT_010274.17
Ensembl:   ENSG00000170776
Entrez:   11214
Chromosome Mapping:   15q24-q25

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