PrimePCR™ Probe Assay: PRKD3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PRKD3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRKD3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057732
Assay Design:   exonic
Chromosome Location:   2:37483905-37484035question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000379066 ENST00000452104 ENST00000234179

Gene Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement which requires intact phopholipase C gamma and the involvement of other PKC family members. [provided by RefSeq Jul 2008]

Gene Symbol:   PRKD3
Gene Name:   protein kinase D3
Aliases:   EPK2, PKC-NU, PKD3, PRKCN, nPKC-NU
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000115825
Entrez:   23683
Chromosome Mapping:   2p21

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