PrimePCR™ Probe Assay: RASGRP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RASGRP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RASGRP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057947
Assay Design:   exonic
Chromosome Location:   11:64507177-64507492question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000377494 ENST00000394432 ENST00000377497 ENST00000354024 ENST00000431822

Gene Information

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain 2 tandem repeats of EF-hand calcium-binding motifs and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RASGRP2
Gene Name:   RAS guanyl releasing protein 2 (calcium and DAG-regulated)
Aliases:   CALDAG-GEFI, CDC25L
RefSeq:   NC_000011.9 NG_013018.1 NT_167190.1 NG_007574.1
Ensembl:   ENSG00000068831
Entrez:   10235
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.230000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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