PrimePCR™ Probe Assay: GUCY1A3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GUCY1A3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GUCY1A3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057998
Assay Design:   exonic
Chromosome Location:   4:156634508-156634652question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000506455 ENST00000511108 ENST00000511507 ENST00000455639 ENST00000393832 ENST00000296518 ENST00000513574

Gene Information

Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3'5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq Jan 2012]

Gene Symbol:   GUCY1A3
Gene Name:   guanylate cyclase 1, soluble, alpha 3
Aliases:   GC-SA3, GUC1A3, GUCA3, GUCSA3, GUCY1A1
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164116
Entrez:   2982
Chromosome Mapping:   4q31.3-q33,4q31.1-q31.2

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