PrimePCR™ Probe Assay: NCOA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NCOA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NCOA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058096
Assay Design:   exonic
Chromosome Location:   2:24914383-24914496question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000406961 ENST00000405141 ENST00000407230 ENST00000395856 ENST00000348332 ENST00000538539 ENST00000288599

Gene Information

The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   NCOA1
Gene Name:   nuclear receptor coactivator 1
Aliases:   F-SRC-1, KAT13A, MGC129719, MGC129720, RIP160, SRC1, bHLHe42, bHLHe74
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000084676
Entrez:   8648
Chromosome Mapping:   2p23

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