PrimePCR™ Probe Assay: ITPR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ITPR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ITPR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058135
Assay Design:   exonic
Chromosome Location:   3:4683798-4683918question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000357086 ENST00000456211 ENST00000443694 ENST00000302640 ENST00000354582 ENST00000423119 ENST00000544951

Gene Information

This gene encodes an intracellular receptor for inositol 145-trisphosphate. Upon stimulation by inositol 145-trisphosphate this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15 a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq Nov 2009]

Gene Symbol:   ITPR1
Gene Name:   inositol 1,4,5-trisphosphate receptor, type 1
Aliases:   DKFZp313E1334, DKFZp313N1434, INSP3R1, IP3R, IP3R1, SCA15, SCA16
RefSeq:   NC_000003.11 NT_022517.18 NG_016144.1
Ensembl:   ENSG00000150995
Entrez:   3708
Chromosome Mapping:   3p26.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.560000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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