PrimePCR™ Probe Assay: CHRNA4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CHRNA4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CHRNA4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026313
Assay Design:   Intron-spanning
Chromosome Location:   20:61978153-61981052question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000370263 ENST00000370258 ENST00000539366

Gene Information

This gene encodes a nicotinic acetylcholine receptor which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq Feb 2012]

Gene Symbol:   CHRNA4
Gene Name:   cholinergic receptor, nicotinic, alpha 4
Aliases:   BFNC, EBN, EBN1, FLJ95812, NACHR, NACHRA4, NACRA4
RefSeq:   NC_000020.10 NG_011931.1 NT_011333.6
Ensembl:   ENSG00000101204
Entrez:   1137
Chromosome Mapping:   20q13.2-q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.630000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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