PrimePCR™ Probe Assay: FASN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FASN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FASN, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026813
Assay Design:   Intron-spanning
Chromosome Location:   17:80051600-80053255question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000306749 ENST00000545909

Gene Information

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA in the presence of NADPH into long-chain saturated fatty acids. In some cancer cell lines this protein has been found to be fused with estrogen receptor-alpha (ER-alpha) in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq Jul 2008]

Gene Symbol:   FASN
Gene Name:   fatty acid synthase
Aliases:   FAS, MGC14367, MGC15706, OA-519, SDR27X1
RefSeq:   NC_000017.10 NT_010663.15
Ensembl:   ENSG00000169710
Entrez:   2194
Chromosome Mapping:   17q25

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