PrimePCR™ Probe Assay: PPP1CA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PPP1CA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPP1CA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027040
Assay Design:   Intron-spanning
Chromosome Location:   11:67167100-67168272question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000312989 ENST00000376745 ENST00000358239 ENST00000451458

Gene Information

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes such as cell division glycogen metabolism muscle contractility protein synthesis and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PPP1CA
Gene Name:   protein phosphatase 1, catalytic subunit, alpha isozyme
Aliases:   MGC15877, MGC1674, PP-1A, PP1alpha, PPP1A
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000172531
Entrez:   5499
Chromosome Mapping:   11q13

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