PrimePCR™ Probe Assay: NOS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NOS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NOS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027049
Assay Design:   Intron-spanning
Chromosome Location:   12:117703227-117705895question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000317775 ENST00000338101 ENST00000397605 ENST00000541241 ENST00000344089

Gene Information

The protein encoded by this gene belongs to the family of nitric oxide synthases which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes including neurotransmission and antimicrobial and antitumoral activities. In the brain and peripheral nervous system nitric oxide displays many properties of a neurotransmitter and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases neural regulation of smooth muscle including peristalsis and penile erection. This protein is ubiquitously expressed with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq Feb 2011]

Gene Symbol:   NOS1
Gene Name:   nitric oxide synthase 1 (neuronal)
Aliases:   IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS
RefSeq:   NC_000012.11 NG_011991.2 NT_009775.17
Ensembl:   ENSG00000089250
Entrez:   4842
Chromosome Mapping:   12q24.2-q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.700000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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