PrimePCR™ Probe Assay: AXIN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AXIN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AXIN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027462
Assay Design:   Intron-spanning
Chromosome Location:   16:364629-396210question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000262320 ENST00000354866

Gene Information

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli catenin beta-1 glycogen synthase kinase 3 beta protein phosphate 2 and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein alone and in a complex with other proteins has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma hepatoblastomas ovarian endometriod adenocarcinomas and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Dec 2010]

Gene Symbol:   AXIN1
Gene Name:   axin 1
Aliases:   AXIN, MGC52315
RefSeq:   NC_000016.9 NG_012267.1 NT_010393.16
Ensembl:   ENSG00000103126
Entrez:   8312
Chromosome Mapping:   16p13.3

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