PrimePCR™ Probe Assay: MAP2K1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAP2K1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAP2K1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028077
Assay Design:   Intron-spanning
Chromosome Location:   15:66774149-66777409question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000307102 ENST00000425818

Gene Information

The protein encoded by this gene is a member of the dual specificity protein kinase family which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases also known as extracellular signal-regulated kinases (ERKs) act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway this kinase is involved in many cellular processes such as proliferation differentiation transcription regulation and development. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP2K1
Gene Name:   mitogen-activated protein kinase kinase 1
Aliases:   MAPKK1, MEK1, MKK1, PRKMK1
RefSeq:   NC_000015.9 NG_008305.1 NT_010194.17
Ensembl:   ENSG00000169032
Entrez:   5604
Chromosome Mapping:   15q22.1-q22.33

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