PrimePCR™ Probe Assay: APC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: APC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: APC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028348
Assay Design:   Intron-spanning
Chromosome Location:   5:112151192-112154727question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000507379 ENST00000257430 ENST00000508376 ENST00000512211 ENST00000457016

Gene Information

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion transcriptional activation and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP) an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq Jul 2008]

Gene Symbol:   APC
Gene Name:   adenomatous polyposis coli
Aliases:   BTPS2, DP2, DP2.5, DP3, GS
RefSeq:   NC_000005.9 NG_008481.4 NT_034772.6
Ensembl:   ENSG00000134982
Entrez:   324
Chromosome Mapping:   5q21-q22

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