PrimePCR™ Probe Assay: WNT5A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WNT5A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WNT5A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028356
Assay Design:   Intron-spanning
Chromosome Location:   3:55504527-55508409question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000474267 ENST00000497027 ENST00000264634 ENST00000536765

Gene Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq Jan 2012]

Gene Symbol:   WNT5A
Gene Name:   wingless-type MMTV integration site family, member 5A
Aliases:   hWNT5A
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000114251
Entrez:   7474
Chromosome Mapping:   3p21-p14

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