PrimePCR™ Probe Assay: CTNND1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CTNND1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CTNND1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028369
Assay Design:   Intron-spanning
Chromosome Location:   11:57571152-57572183question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000524630 ENST00000529919 ENST00000529526 ENST00000426142 ENST00000399050 ENST00000361391 ENST00000361332 ENST00000532463 ENST00000529986 ENST00000358694 ENST00000532787 ENST00000533667 ENST00000532649 ENST00000528621 ENST00000530748 ENST00000428599 ENST00000527467 ENST00000528232 ENST00000531014 ENST00000526772 ENST00000529873 ENST00000525902 ENST00000532844 ENST00000526357 ENST00000530094 ENST00000415361 ENST00000532245 ENST00000534579 ENST00000526938 ENST00000399039 ENST00000360682 ENST

Gene Information

This gene encodes a member of the Armadillo protein family which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq Dec 2010]

Gene Symbol:   CTNND1
Gene Name:   catenin (cadherin-associated protein), delta 1
Aliases:   CAS, CTNND, KIAA0384, P120CAS, P120CTN, p120, p120(CAS), p120(CTN)
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000198561
Entrez:   1500
Chromosome Mapping:   11q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.090000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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