PrimePCR™ Probe Assay: LAT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LAT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LAT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029391
Assay Design:   Intron-spanning
Chromosome Location:   16:28997997-29000898question
Amplicon Length:   155
Splice Variants Targeted:   ENST00000395461 ENST00000360872 ENST00000395456 ENST00000454369 ENST00000354453

Gene Information

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   LAT
Gene Name:   linker for activation of T cells
Aliases:   LAT1, pp36
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000213658
Entrez:   27040
Chromosome Mapping:   16p11.2

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