PrimePCR™ Probe Assay: PIM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PIM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PIM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029454
Assay Design:   Intron-spanning
Chromosome Location:   6:37140867-37141837question
Amplicon Length:   180
Splice Variants Targeted:   ENST00000373509

Gene Information

The protein encoded by this gene belongs to the Ser/Thr protein kinase family and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells contributing to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805 1825810).[provided by RefSeq Aug 2011]

Gene Symbol:   PIM1
Gene Name:   pim-1 oncogene
Aliases:   PIM
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000137193
Entrez:   5292
Chromosome Mapping:   6p21.2

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