PrimePCR™ Probe Assay: EFNB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EFNB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EFNB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031362
Assay Design:   Intron-spanning
Chromosome Location:   13:107145671-107147300question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000245323

Gene Information

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships ephrins are divided into the ephrin-A (EFNA) class which are anchored to the membrane by a glycosylphosphatidylinositol linkage and the ephrin-B (EFNB) class which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq Jul 2008]

Gene Symbol:   EFNB2
Gene Name:   ephrin-B2
Aliases:   EPLG5, HTKL, Htk-L, LERK5, MGC126226, MGC126227, MGC126228
RefSeq:   NC_000013.10 NT_009952.14
Ensembl:   ENSG00000125266
Entrez:   1948
Chromosome Mapping:   13q33

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