PrimePCR™ Probe Assay: EPHB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EPHB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EPHB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031643
Assay Design:   Intron-spanning
Chromosome Location:   3:134644658-134670264question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000460895 ENST00000398015 ENST00000497173 ENST00000473867 ENST00000474732

Gene Information

Ephrin receptors and their ligands the ephrins mediate numerous developmental processes particularly in the nervous system. Based on their structures and sequence relationships ephrins are divided into the ephrin-A (EFNA) class which are anchored to the membrane by a glycosylphosphatidylinositol linkage and the ephrin-B (EFNB) class which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq Jul 2008]

Gene Symbol:   EPHB1
Gene Name:   EPH receptor B1
Aliases:   ELK, EPHT2, FLJ37986, Hek6, NET
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000154928
Entrez:   2047
Chromosome Mapping:   3q21-q23

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