PrimePCR™ Probe Assay: CYP2C8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CYP2C8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYP2C8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033001
Assay Design:   Intron-spanning
Chromosome Location:   10:96802761-96805641question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000371270 ENST00000535898 ENST00000535868 ENST00000539050

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics including the anticonvulsive drug mephenytoin benzo(a)pyrene 7-ethyoxycoumarin and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq Nov 2010]

Gene Symbol:   CYP2C8
Gene Name:   cytochrome P450, family 2, subfamily C, polypeptide 8
Aliases:   CPC8, CYPIIC8, MP-12/MP-20
RefSeq:   NC_000010.10 NG_007972.1 NT_030059.13
Ensembl:   ENSG00000138115
Entrez:   1558
Chromosome Mapping:   10q23.33

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