PrimePCR™ Probe Assay: AP2M1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AP2M1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AP2M1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033446
Assay Design:   Intron-spanning
Chromosome Location:   3:183899991-183900615question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000382456 ENST00000411763 ENST00000292807 ENST00000439647 ENST00000442686 ENST00000540821 ENST00000539646

Gene Information

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   AP2M1
Gene Name:   adaptor-related protein complex 2, mu 1 subunit
Aliases:   AP50, CLAPM1, mu2
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000161203
Entrez:   1173
Chromosome Mapping:   3q28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.450000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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