PrimePCR™ Probe Assay: PIK3CA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PIK3CA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PIK3CA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033870
Assay Design:   Intron-spanning
Chromosome Location:   3:178941943-178942560question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000263967

Gene Information

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit which uses ATP to phosphorylate PtdIns PtdIns4P and PtdIns(45)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq Jul 2008]

Gene Symbol:   PIK3CA
Gene Name:   phosphoinositide-3-kinase, catalytic, alpha polypeptide
Aliases:   MGC142161, MGC142163, PI3K, p110-alpha
RefSeq:   NC_000003.11 NG_012113.1 NT_005612.16
Ensembl:   ENSG00000121879
Entrez:   5290
Chromosome Mapping:   3q26.3

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