PrimePCR™ Probe Assay: PPP2R1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PPP2R1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPP2R1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040778
Assay Design:   Intron-spanning
Chromosome Location:   11:111614202-111622955question
Amplicon Length:   157
Splice Variants Targeted:   ENST00000503265 ENST00000571959 ENST00000571902 ENST00000576276 ENST00000575443 ENST00000573946 ENST00000274513 ENST00000433282 ENST00000420621 ENST00000373816 ENST00000456530 ENST00000301178 ENST00000324306 ENST00000358661 ENST00000002829 ENST00000434342 ENST00000343932 ENST00000408936 ENST00000373782 ENST00000309989 ENST00000259371 ENST00000532312 ENST00000265755 ENST00000455841 ENST00000424337 ENST00000476977 ENST00000470715 ENST00000344683 ENST00000373544 ENST00000377411 ENST00000339656 ENST

Gene Information

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme which is composed of a catalytic subunit and a constant regulatory subunit that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq Apr 2010]

Gene Symbol:   PPP2R1B
Gene Name:   protein phosphatase 2, regulatory subunit A, beta
Aliases:   MGC26454, PP2A-Abeta, PR65B
RefSeq:   NC_000011.9 NT_033899.8 NG_012117.1
Ensembl:   ENSG00000137713
Entrez:   5519
Chromosome Mapping:   11q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.430000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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