PrimePCR™ Probe Assay: Gria4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Gria4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gria4, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0041907
Assay Design:   exonic
Chromosome Location:   9:4537676-4537782question
Amplicon Length:   77
Splice Variants Targeted:   ENSMUST00000027020 ENSMUST00000063508 ENSMUST00000163309

Gene Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms which may vary in their signal transduction properties. [provided by RefSeq Jul 2008]

Gene Symbol:   Gria4
Gene Name:   Glutamate receptor, ionotropic, AMPA4 (alpha 4)
Aliases:   GluA4, GluR-D, Glur-4, Glur4, Gluralpha4, spkw1
RefSeq:   NC_000075.6 NT_039471.8
Ensembl:   ENSMUSG00000025892
Entrez:   14802
Chromosome Mapping:   9 A1|9 8.0 cM

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