PrimePCR™ Probe Assay: Fgfr3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Fgfr3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Fgfr3, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042662
Assay Design:   exonic
Chromosome Location:   5:33729735-33729847question
Amplicon Length:   83
Splice Variants Targeted:   ENSMUST00000067150 ENSMUST00000164207 ENSMUST00000087820 ENSMUST00000155002 ENSMUST00000169212 ENSMUST00000114411 ENSMUST00000171509

Gene Information

This gene encodes a member of the fibroblast growth factor receptor family. Members of this family are highly conserved proteins that differ from one another in their ligand affinities and tissue distribution. A representative protein consists of an extracellular region composed of three immunoglobulin-like domains a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors setting in motion a cascade of downstream signals ultimately influencing mitogenesis and differentiation. This family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene may be associated with craniosynostosis and multiple types of skeletal dysplasia. A pseudogene of this gene is located on chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Apr 2011]

Gene Symbol:   Fgfr3
Gene Name:   Fibroblast growth factor receptor 3
Aliases:   CD333, FR3, Fgfr-3, Flg-2, HBGFR, Mfr3, sam3
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000054252
Entrez:   14184
Chromosome Mapping:   5 B|5 20.0 cM

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