PrimePCR™ Probe Assay: Set, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Set, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Set, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043223
Assay Design:   exonic
Chromosome Location:   2:30070230-30070321question
Amplicon Length:   62
Splice Variants Targeted:   ENSMUST00000102683 ENSMUST00000093048 ENSMUST00000093046 ENSMUST00000095254 ENSMUST00000103121 ENSMUST00000046944 ENSMUST00000169335 ENSMUST00000118869 ENSMUST00000044551 ENSMUST00000064099 ENSMUST00000080553 ENSMUST00000086112 ENSMUST00000077517 ENSMUST00000092888 ENSMUST00000118282 ENSMUST00000091967 ENSMUST00000025239 ENSMUST00000059857 ENSMUST00000121357 ENSMUST00000119373 ENSMUST00000118159 ENSMUST00000045682 ENSMUST00000165104 ENSMUST00000009550 ENSMUST00000058229 ENSMUST00000142395 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Set
Gene Name:   SET nuclear oncogene
Aliases:   2610030F17Rik, 5730420M11Rik, AA407739, I-2PP2A, StF-IT-1, TAF-I
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000054766
Entrez:   56086
Chromosome Mapping:   2 B|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.700000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download