PrimePCR™ Probe Assay: Psme2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Psme2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Psme2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043659
Assay Design:   exonic
Chromosome Location:   14:55587654-55588060question
Amplicon Length:   77
Splice Variants Targeted:   ENSMUST00000047480 ENSMUST00000072800 ENSMUST00000024810 ENSMUST00000167591 ENSMUST00000092070 ENSMUST00000161807 ENSMUST00000111378 ENSMUST00000020586 ENSMUST00000092915 ENSMUST00000071972 ENSMUST00000081921 ENSMUST00000106239 ENSMUST00000062306 ENSMUST00000104958 ENSMUST00000178124 ENSMUST00000099557 ENSMUST00000110853 ENSMUST00000084787

Gene Information

Description Not Available

Gene Symbol:   Psme2
Gene Name:   Proteasome (prosome, macropain) 28 subunit, beta
Aliases:   AA589371, AI788882, PA28b
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000079197
Entrez:   19188
Chromosome Mapping:   14 C2-D1|14 22.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.890000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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