PrimePCR™ Probe Assay: Hck, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Hck, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hck, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053374
Assay Design:   exonic
Chromosome Location:   2:153144441-153144544question
Amplicon Length:   74
Splice Variants Targeted:   ENSMUST00000003370 ENSMUST00000109799

Gene Information

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage including a non-AUG (CUG) codon results in the production of two different isoforms that have different subcellular localization. [provided by RefSeq Feb 2010]

Gene Symbol:   Hck
Gene Name:   Hemopoietic cell kinase
Aliases:   AI849071, Bmk
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000003283
Entrez:   15162
Chromosome Mapping:   2 H1|2 86.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.980000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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