PrimePCR™ Probe Assay: Tyms, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Tyms, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Tyms, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0054588
Assay Design:   exonic
Chromosome Location:   5:30059068-30059200question
Amplicon Length:   103
Splice Variants Targeted:   ENSMUST00000026846

Gene Information

This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 510-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR (PMID: 3022294 3444407). The stop codon in these transcripts is UAA compared to the UAG found in the genome and longer transcripts as the polyA site is located within the stop codon (PMID: 3444407 2157203). A related pseudogene has been identified on chromosome 10. [provided by RefSeq Mar 2010]

Gene Symbol:   Tyms
Gene Name:   Thymidylate synthase
Aliases:   Ts
RefSeq:   NC_000071.6 NT_165760.3
Ensembl:   ENSMUSG00000025747
Entrez:   22171
Chromosome Mapping:   5 B1|5 18.2 cM

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