PrimePCR™ Probe Assay: Ptges2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: Ptges2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Ptges2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0054962
Assay Design:   exonic
Chromosome Location:   2:32396022-32396111question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000028162

Gene Information

The protein encoded by this gene is a Golgi membrane-associated prostaglandin E synthase candidate which is capable of catalyzing the conversion of prostaglandin H2 to prostaglandin E2 in vitro. However a study using mice deficient of this gene suggests that this enzyme does not contribute to prostaglandin E2 biosynthesis in vivo. This protein is synthesized as a Golgi membrane-bound protein but its N-terminal hydrophobic region is cleaved off during protein maturation to produce the predominant soluble truncated form that still retains the enzyme activity. This soluble protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE) possibly via an interaction with CAAAT/enhancer-binding protein-beta. [provided by RefSeq Oct 2009]

Gene Symbol:   Ptges2
Gene Name:   Prostaglandin E synthase 2
Aliases:   0610038H10Rik, C79137, Gbf1, Mpges2, Pges2
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000026820
Entrez:   96979
Chromosome Mapping:   2 B|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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