PrimePCR™ Probe Assay: Socs1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Socs1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Socs1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0057945
Assay Design:   exonic
Chromosome Location:   16:10784129-10784278question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000049572 ENSMUST00000178114 ENSMUST00000038099

Gene Information

Description Not Available

Gene Symbol:   Socs1
Gene Name:   Suppressor of cytokine signaling 1
Aliases:   Cish1, Cish7, JAB, SOCS-1, SSI-1
RefSeq:   NC_000082.6 NT_039624.8
Ensembl:   ENSMUSG00000038037
Entrez:   12703
Chromosome Mapping:   16 A1|16 3.4 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 35.800000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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