PrimePCR™ Probe Assay: Lig1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Lig1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Lig1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0058899
Assay Design:   exonic
Chromosome Location:   7:13282456-13285260question
Amplicon Length:   61
Splice Variants Targeted:   ENSMUST00000148471 ENSMUST00000156525 ENSMUST00000146998 ENSMUST00000123846 ENSMUST00000123025 ENSMUST00000147735 ENSMUST00000177588 ENSMUST00000098814 ENSMUST00000165964

Gene Information

Description Not Available

Gene Symbol:   Lig1
Gene Name:   Ligase I, DNA, ATP-dependent
Aliases:   AL033288, LigI
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000056394
Entrez:   16881
Chromosome Mapping:   7 A1|7 4.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.180000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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