PrimePCR™ Probe Assay: Ldha, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Ldha, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Ldha, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0060053
Assay Design:   exonic
Chromosome Location:   7:46847386-46847691question
Amplicon Length:   69
Splice Variants Targeted:   ENSMUST00000147535 ENSMUST00000048209 ENSMUST00000092621 ENSMUST00000005051

Gene Information

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Two transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq Sep 2009]

Gene Symbol:   Ldha
Gene Name:   Lactate dehydrogenase A
Aliases:   Ldh1, Ldhm, l7R2
RefSeq:   NC_000073.6 NT_187035.1
Ensembl:   ENSMUSG00000063229
Entrez:   16828
Chromosome Mapping:   7 B4|7 23.5 cM

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