PrimePCR™ Probe Assay: Gpx2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Gpx2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gpx2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0028143
Assay Design:   Intron-spanning
Chromosome Location:   12:76792948-76795310question
Amplicon Length:   180
Splice Variants Targeted:   ENSMUST00000082431

Gene Information

This gene product belongs to the glutathione peroxidase family which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Knockout studies in mice indicate that mRNA expression and activity of this enzyme are induced by luminal microflora suggesting a role for this peroxidase in preventing inflammation in the GI tract. [provided by RefSeq Jul 2008]

Gene Symbol:   Gpx2
Gene Name:   Glutathione peroxidase 2
Aliases:   GI-GPx, GPx-GI, GSHPx-2, GSHPx-GI
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000042808
Entrez:   14776
Chromosome Mapping:   12 C3|12 36.0 cM

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