PrimePCR™ Probe Assay: Trp63, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Trp63, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Trp63, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0030077
Assay Design:   Intron-spanning
Chromosome Location:   16:25868172-25871065question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000115310 ENSMUST00000040231 ENSMUST00000115306 ENSMUST00000115305 ENSMUST00000065523 ENSMUST00000115308

Gene Information

This gene encodes tumor protein p63 a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include tumor proteins p53 p63 and p73 which have high sequence similarity to one another. This similarity allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways including direct and indirect protein interactions. This results in mutual regulation of target gene promoters. Tumor protein p63 -/- mice have several developmental defects which include the lack of limbs and other tissues such as teeth and mammary glands which develop as a result of interactions between mesenchyme and epithelium. Both alternative splicing and the use of alternative promoters result in multiple transcript variants encoding different protein isoforms.[provided by RefSeq Dec 2009]

Gene Symbol:   Trp63
Gene Name:   Transformation related protein 63
Aliases:   AI462811, Ket, P51/P63, P63, P73l, Tp63, Trp53rp1
RefSeq:   NC_000082.6 NT_039624.8
Ensembl:   ENSMUSG00000022510
Entrez:   22061
Chromosome Mapping:   16 B1|16 19.3 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.830000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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