PrimePCR™ Probe Assay: PPIA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PPIA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPIA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041342
Assay Design:   exonic
Chromosome Location:   7:44841013-44841175question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000355968 ENST00000489459 ENST00000468812

Gene Information

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins including p55 gag Vpr and capsid protein and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   PPIA
Gene Name:   peptidylprolyl isomerase A (cyclophilin A)
Aliases:   CYPA, CYPH, MGC117158, MGC12404, MGC23397
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000196262
Entrez:   5478
Chromosome Mapping:   7p13

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