PrimePCR™ Probe Assay: UGT1A10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: UGT1A10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UGT1A10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041551
Assay Design:   exonic
Chromosome Location:   2:234545395-234545532question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000373450 ENST00000344644 ENST00000373445 ENST00000373426

Gene Information

This gene encodes a UDP-glucuronosyltransferase an enzyme of the glucuronidation pathway that transforms small lipophilic molecules such as steroids bilirubin hormones and drugs into water-soluble excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site and is regulated by its own promoter. The enzyme encoded by this gene has glucuronidase activity on mycophenolic acid coumarins and quinolines. [provided by RefSeq Jul 2008]

Gene Symbol:   UGT1A10
Gene Name:   UDP glucuronosyltransferase 1 family, polypeptide A10
Aliases:   UDPGT, UGT1J
RefSeq:   NG_009254.1 NC_000002.11 NG_002601.2 NT_005120.16
Ensembl:   ENSG00000242515
Entrez:   54575
Chromosome Mapping:   2q37

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