PrimePCR™ Probe Assay: ITGA5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ITGA5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ITGA5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053200
Assay Design:   exonic
Chromosome Location:   12:54789659-54789794question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000293379 ENST00000547197

Gene Information

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq Jul 2008]

Gene Symbol:   ITGA5
Gene Name:   integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Aliases:   CD49e, FNRA, VLA5A
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000161638
Entrez:   3678
Chromosome Mapping:   12q11-q13

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